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. Author manuscript; available in PMC: 2012 Sep 22.
Published in final edited form as: J Med Chem. 2011 Aug 23;54(18):6206–6214. doi: 10.1021/jm200479c

Figure 3.

Figure 3

Cell-based characterization of 19. A) TR-FRET analysis of c-Jun phosphorylation upon TNF-αstimulation of B16-F10 cells in the presence of increasing 19 (closed triangles), 21 (inverted triangles) and 11 (closed squares). B) Inhibitory effect of 19 on phospho-c-Jun upon anisomycin stimulation of HEK293T cell. C) Compounds 19 and 11 effect on TNF-α and IL-1β levels after 5 hours of exposure to LPS as compared to vehicle control. Results shown as percent of vehicle control ± S.D. (n = 3). Cytokine production was measured directly from cell culture medium by a sandwich immunoassay (Meso Scale Discovery).