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Western blots show the results of co-immunoprecipitation experiments in four separate batches of preglomerular vascular smooth muscle cells using the Santa Cruz BT: ExactaCruz IP kit. The immunoprecipitation step was conducted with an antibody against either the inositol 1,4,5-trisphosphate receptor (IP3R; panel A) or calmodulin (panel B) or with non-immune serum (panel C). Immunoprecipitates were analyzed by Western blotting using an anti-RACK1 antibody for detection.
