Fig. 1.

Chromosome painting in PMCs of B. distachyon at late zygotene/pachytene. a–e Painting using BAC pools spanning short (green) and long (red) arms of a given chromosome. The ideograms next to the photographs indicate the approximate physical positions of the pools used. CEN = centromere. a chromosome Bd1; b chromosome Bd2; c chromosome Bd3; d chromosome Bd4; e chromosome Bd5; f painting of the short arm of chromosome Bd1 with 70 BAC clones arranged into seven alternating pools. White arrows indicate additional unexpected signals. The red arrow in c indicates the position of an unpaired chromosome region on Bd3. All chromosomes were counterstained with DAPI. Bars = 5 μm