Fig. 3.

Identification of chromosome territories in interphase nuclei of B. distachyon anther tissue using chromosome painting. a–b Simultaneous painting of chromosomes Bd1 (green) and Bd2 (red). a Interphase nucleus showing association of homologous chromosomes while territories occupied by heterologues remain separated; b interphase nucleus showing separation of all four chromosome territories; c–e painting of the short (red) and long (green) arm of chromosome Bd1; c association of territories of both arms; d association of territories of the short arm; e separation of territories occupied by chromosome Bd1; f chromosome painting of interphase nuclei with seven alternating BAC pools specific to different regions of Bd1 short arm: the result is visible as a mixture of red and green signals and the territories occupied by particular pools are not discernible. The drawings next to the photographs indicate approximate positions of the BAC pools used for painting. CEN = centromere. All chromosomes were counterstained with DAPI. Bars = 5 μm