. 2011 Sep 14;7(8):1101–1113. doi: 10.7150/ijbs.7.1101

Table 1.

Primers used in this study

Gene	Primer symbol	Primer sequence (5'-3')	Tm (℃)	Used for
MMP19	MMP19.L	ACAGGGTCCGTATGAGGCA	65	cDNA cloning
MMP19	MMP19.R	GGAAATCCGAGGCTCAACG	65
MMP19	5'RACE.MMP19.R	GGTTCAAGATGCGGAAGGTCAGGTGC
MMP19	3'RACE.MMP19.L	CCACGCCGTTGGATGGGGATACTTC
MMP19	3'RACE nest	TCTCCACCACCCACTCATTC
MMP23B	MMP23B-CDS.L	GACGCCGCTACACGCTGACC	65	cDNA cloning
MMP23B	MMP23B-CDS.R	CGCGGACCCCCCAGGAGTA	65
MMP23B	MIB2.3003	GCTACCGGCAGATGGAGGAACGC
MMP23B	MMP23B.258	GGTCAGCGTGTAGCGGCGTCTTC
MMP23B	3'RACE.MMP23B.R	TACCCCGTCAACCACACCGACTGCC
MMP23B	3'RACE nest	GACTTCTGCTACGAGTTCCC
MMP19	19.116	GGCTTCCTACTCCCCATGAC	60	Intron 1Intron 2
MMP19	19.336	CTCATACGGACCCTTGTGGC	60	Intron 1Intron 2
MMP19	19.311	GATGATGCCACAAGGGTCCG	58	Intron 3
MMP19	19.601	GTTATTGGAGCAGTATGGGC	58	Intron 3
MMP19	19.501	GTGGCTCCCTTGACTTTCCG	56	Intron 4
MMP19	19.775	CTGGGTGTATCGGGAGTG	56	Intron 4
MMP19	19.715	CATCATTGCAGCCCACGAAC	59	Intron 5,6,7
MMP19	19.1085	CTTCCCAAAGGGCAGACAC	59	Intron 5,6,7
MMP19	19.1031	ACCGTGACAGATTCAGGGC	59	Intron 8
MMP19	19.2114	AATCCGAGGCTCAACGACG	59	Intron 8
MMP19	19map1	TGCTCAACGGCACCCTAC	58	mapping
MMP19	19map2	AGGAAGGCGGTCAGAGTC	58
MMP23B	23map1	CAAAGAGCGGCACCGAAAGC	60
MMP23B	23map2	GTGGTCCCAACGTAGCCTG	60
MMP19	MMP19V1-F	TCGGACACAATGGATTCACTTC		expression analyses
MMP19	MMP19V1-R	GCCCGGAGACATCTTGAAATT
MMP19	MMP19V1-P	FAM-CGTAGCGCCACACCTTGTCTCCCT-TAMRA
MMP23B	MMP23B-F	TCGCAGGACGAGCTGTGG
MMP23B	MMP23B-R	GCGTCACAGAAGCCCCTC
MMP23B	MMP23B-P	FAM-CACACGAACAGCCGGTCCAGG-TAMRA
β-actin	ATCB-F	TCCAGAGGCGCTCTTCCA
β-actin	ATCB-R	CGCACTTCATGATCGAGTTGA
β-actin	ATCB-P	FAM-CCTCCTTCCTGGGCATGGAGTCCT-TAMRA
MMP19	19mor3023:exon 5	ACTCTGACCGCCTTCCTC	60	SNP genotyping
MMP19	19mor3367:exon 5	TCAAGCCCGAAGAGGTGC	60
MMP23B	23mor2.1:exon 3	AGAGCGGCACCGAAAGCG	60
MMP23B	23mor2.2:exon 3	CTGCTTGACCCCAGACCC	60
MMP23B	23mor260:exon 4	CGGCGGCATCCACTTTGACG	60
MMP23B	23mor2.2:exon 4	CTGCTTGACCCCAGACCC	60