Figure 7.

Effects of K_Ca inhibition by apamin + charybdotoxin on ACh, 2-furoyl-LIGRLO-amide (2fly)- and trypsin-induced relaxations of db/db and C57BL/6J (C57) mesenteric arteries. Columns are the mean ± SE (n = number of mice) for (A) ACh- (B) 2fly- and (C) trypsin-induced relaxations of C57 and db/db second order mesenteric arteries contracted submaximally by U46619. Arteries were exposed to inhibitors [100 µM l-NAME, 1 µM apamin, 50 nM charybdotoxin (ChTx)] for 30 min prior to U46619. *P < 0.05, compared with C57 control (untreated); **P < 0.05, compared with same strain control (untreated); ***P < 0.05, compared with same strain control (untreated) and same strain l-NAME-treated, two-way anova (strain × treatment) and Bonferroni post hoc.^aP < 0.05, main effect of strain contributed 2.5% of total variation, two-way anova. In (C) control C57 vs. control db/db, and control db/db vs. l-NAME-treated db/db were not different, Bonferroni post hoc. In (B) and (C), the interaction between strain and treatment was not significant (P > 0.05).