Skip to main content
. Author manuscript; available in PMC: 2012 Sep 21.
Published in final edited form as: J Am Chem Soc. 2011 Aug 25;133(37):14492–14495. doi: 10.1021/ja204716f

Figure 4.

Figure 4

FTICR-MS analysis of cryptophycin products from the reaction of unit C monomethyl chain elongation intermediate (3-amino-2(R)-methylpropionyl, 3) with l/d-2HIC, ATP, CrpD-M2, and Crp TE. EICs are presented at +/− 15 ppm as time versus absolute signal. Inset mass spectra are time averaged over the 1 minute elution window corresponding to the asterisk in the extracted ion chromatogram. Inset mass spectra are presented as m/z versus absolute signal. Monoisotopic mass MH+ and the experimental mass error in ppm are reported. Reactions with l-2HIC and d-2HIC monitor the formation of cyclic (A and C) and linear products (B and D). No enzyme control reactions for cyclic (E) and linear product formation (F) are provided.