Figure 1.
Varying strengths of different toxin lines in ablating the eye. (A) Control: y1 w67c23 x eyeless-Gal4. (B) Crosses between eyeless-Gal4 and the less effective UAS-M2(H37A)-1LE toxin line resulted in a rate of eclosion of 1:10. The progeny from this cross displayed the total absence of eyes, reduced eyes, and/or cyclopia. (C) In contrast, crosses with both the highly efficient toxin lines UAS-M2(H37A)-3ME and UAS-rpr resulted in flies arresting at different stages of development within pupal casings. Progeny from UAS-M2(H37A)-3ME toxin strain x eyeless-Gal4 cross showed cephalic structure consisting of only the presumptive proboscis still attached to the larval mouth hook (arrowhead). Ablation of cephalic structures were due to the non-specificity of the eyeless driver, as verified by eyeless-Gal4>UAS-GFP screening.