Figure 2.

Increase in size of melanotic masses in third instar larvae. hop^Tum-l MSNF9mo-Gal4>UAS-Red Stinger was crossed with y¹ w^67c23 (A–C), UAS-M2(H37A)-3ME (D–F) and UAS-rpr (G and H). A significant reduction in circulating lamellocytes marked with DsRed was seen in crosses with both UAS-M2(H37A)-3ME and UAS-rpr as shown in (E and H), respectively. In addition, large melanotic masses formed in more than half of all larvae from the UAS-M2(H37A)-3ME crosses (arrowheads in D–F). In contrast, resulting progeny from crosses between UAS-rpr and hop^Tum-l MSNF9-Gal4 UAS-Red Stinger did not display large melanotic masses (G and H). In third instar circulating hemocytes, a reduction in lamellocytes was seen in the progeny from UAS-M2(H37A)-3ME crosses (F) as compared with the control crosses with y¹ w^67c23 (C).