Skip to main content
. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Neurochem. 2011 Aug 22;119(1):189–201. doi: 10.1111/j.1471-4159.2011.07389.x

Figure 3. Anti-Aβ dimer mAbs 3C6 and 4B5 binding to solution-phase Aβ conformers.

Figure 3

Antibody binding to solution-phase Aβ conformers was established in a competition assay whereby mAbs 3C6 (A) and 4B5 (B) binding to plate-immobilized (Aβ1-40S26C)2 was determined in the presence of: freshly-isolated (Aβ1-40S26C)2 (●); (Aβ1-40S26C)2 stored for ~1 month at −80 °C in 25 mM ammonium acetate, pH 8.5, (▼); Freshly-isolated Aβ1-40S26C monomers (□), and WT Aβ1-40 fibrils (◊). Antibody binding was carried out in PBSA containing 1% BSA and 0.05% Tween 20, pH 7.4.