Comparison of the expression level of selected HPA axis genes (CRH, POMC, MC1R, MC2R, CYP11A1, CYP11B1) after UVA, UVB, or UVC irradiation followed by 12 h of cultured human skin (A), and co-cultured HEKn/HEMn after UVB (B). See text for definitions. Values on the x-axis are doses of irradiation: mJ/cm2 for UVB and UVC and J/cm2 for UVA. On the y-axis are provided the mRNA expression levels as a fold change using a comparative ΔΔCT method with β-actin. Real-time RT-PCR presented as means ± SD.