Table 4.
List of primary antibody used for Western blotting or immunohistochemistry
| Antigen | Host | Titer | Source |
|---|---|---|---|
| Corticotropin-releasing hormone, CRH* (PBL rC70) | Rabbit | 1:2,000 | Prof. Wylie Vale, Salk Inst., USA |
| Proconvertase-1, PC1 | Rabbit | 1:1,000 | Dr. Iris Lindberg, USA |
| Adrenocorticotropic hormone, ACTH*,# | Rabbit | 1:500 | Dr. Allen, USA |
| Adrenocorticotropic hormone, ACTH (AFP-6328031) | Rabbit | 1:1,000 | Dr. Parlow, NIDDKD, USA |
| β-Endorphins, β-END* | Rabbit | 1:2,000 | Dr. Allen, USA |
| Cytochrome P-450 side-chain-cleavage, P450scc | Rabbit | 1:1,000 | Prof. Robert Tuckey, Univ. of Western Australia |
| Glucocorticoid receptor, GR (sc-8992) | Rabbit | 1:500 | Santa Cruz Biotech., USA |
| MEL-5 (MA02026) | Mouse | 1:300 | Signet, USA |
| β-Actin-HRP, β-actin# (A3854) | Mouse | 1:10,000 | Sigma, USA |