Fig. 2.

A and B: transforming growth factor (TGF)-β induced a transient upregulation of β-DG expression in activated mHSC/myofibroblasts. Time-course expression of β-DG mRNA in TGF-β-treated activated HSC/myofibroblasts (◊) revealed a transient upregulation that occurred after 6 h (P = 0.037). In control HSC (○), β-DG mRNA increased slowly with time, but values did not reach statistical significance. In platelet-derived growth factor-BB (PDGF-BB)-treated activated mHSC/myofibroblasts (□), changes in mRNA expression were similar to those observed in untreated controls (P = 0.032). Signal intensities of autoradiographies were determined by densitometric analysis. Values were calculated as fold change compared with time zero and are means ± SE of 3 experiments after correcting for loading differences using expression of S14 mRNA as an internal control. *P < 0.05 was considered significant. Statistical analysis was performed using ANOVA. B: representative Northern blots. Each set corresponds to independent experiments and include time zero controls as well as the untreated controls harvested at the same time points as the cultures treated with the corresponding cytokines/growth factors. Act D, actinomycin D.