Fig. 5.
PDGF-BB but not TGF-β shortens the t1/2 of β-DG mRNA by 50%. Activated HSC/myofibroblasts were incubated for 30 min with 10 μg/ml of actinomycin D before the addition of PDGF-BB, TGF-β, or TNF-α. Changes in β-DG mRNA expression were analyzed by Northern analysis of total RNA extracted at the various time points shown. Values are means ± SE of ≥3 independent experiments. The t1/2 of DG mRNA was estimated from the slope of the double exponential curves. Representative Northern blots for these experiments are shown in Fig. 2B.