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. 2011 Jun 9;301(3):G464–G474. doi: 10.1152/ajpgi.00078.2011

Fig. 7.

Fig. 7.

A: β-DG and laminin colocalize to the cell surface of activated HSC/myofibroblasts. Confocal images (2048 × 2048) of control untreated cells taken with ×60 objective (N.A. 1.4) at 0.110-μm pixel resolution, depicting immunolabeling for β-DG (a, red) and laminin (LAM) (b, green). c: Image obtained after merging red and green channels from a and b, respectively. Colocalization was found mainly in the cell body and at the presumptive surface of the cells (arrows). d: Pixels representing colocalization over threshold were extracted and shown in yellow. Scale bar = 21.3 μm. B: PDGF-BB treatment of activated HSC/myofibroblasts enhances the cell surface distribution of β-DG and laminin. PDGF-BB-treated activated HSC/myofibroblasts were immunolabeled with antibodies to β-DG (a, red) and laminin (b, green). This experiment was performed as described in A. c: Merged image of a and b, respectively. Colocalization pixels increased at the presumptive surface of the cells (arrows). d: Pixels representing colocalization over threshold were extracted and shown in yellow; although scattered colocalization was found in the cell body, most colocalization was present mainly within the cell protrusions. Scale bar = 36.4 μm.