Fig. 2.

A: RNA levels of Mybl2, leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5), cyclin B2, cyclin D2, Kruppel-like factor-4 (KLF4), and ALPi in tissue fractions isolated sequentially from the crypt-luminal axis of the human colon were measured by real-time PCR. Values shown are normalized to GAPDH and expressed relative to the level in the fraction at crypt bottom. B: Mybl2, α-tubulin, and villin protein levels in human colon crypt-luminal tissue fractions were measured by Western blotting.