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. 2011 Jun 16;301(3):G498–G507. doi: 10.1152/ajpgi.00164.2011

Fig. 6.

Fig. 6.

A: Western blot depicting nNOSα in WT and DBA varicosities. Note absence of nNOSα in the lane loaded with membrane extracts from DBA varicosities. Samples were boiled before loading. B: in vitro NO production after KCl stimulation in WT and DBA varicosities. Fluorescent NO signal was monitored by preloading the varicosities with the NO indicator DAF-2DA. Note that WT varicosities showed robust NO production after stimulation with 50 mM KCl. In contrast, some DBA varicosities showed only scant DAF-NO signals. Robust signals were seen after preincubation of WT varicosities with DNO. Preincubation of varicosities with l-NAME before KCl stimulation abolished DAF-NO fluorescent signal. Scale bar = 3 μm.