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. 2011 Jun 15;301(3):F622–F633. doi: 10.1152/ajprenal.00134.2011

Fig. 1.

Fig. 1.

Galectin-7 (Gal-7) localization in polarized Madin-Darby canine kidney (MDCK) cells. Polarized MDCK cells were fixed, blocked, permeabilized, and incubated with primary antibodies [mouse anti-Gal-7 and rabbit anti-polycystin-1 (PC-1)], then secondary antibodies, before fixation, staining with TOPRO, and mounting for confocal microscopy. A: distribution of Gal-7 (green) and the nucleus (blue) at the apical pole (a–c) and along the lateral surface (d–f) of MDCK cells. Arrows indicate the localization of Gal-7 on the primary cilia. B: distribution of Gal-7 (green), PC-1 (a marker of cilia; red), and the nucleus (blue) at the apical pole (a–d) of MDCK cells. Arrows indicate colocalization between Gal-7 and PC-1 on the primary cilia. C: distribution of Gal-7 (green) and the nucleus (blue) at the apical pole (a–c) of MDCK cells grown overnight with 100 mM lactose. Single representative merged images (Ac, Af, Bd, and Cc,) and XZ sections (Ag, Be, and Cd) are shown. Scale bar = 10 μm.