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. 2011 Jun 8;301(3):F544–F553. doi: 10.1152/ajprenal.00709.2010

Fig. 2.

Fig. 2.

Effects of cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor-172 and flufenamic acid (FFA) on ATP-induced Isc in mIMCD-K2 cells. A: representative trace of Isc in response to sequential addition of amiloride (Am; apical addition at 10−5 M), CFTR inhibitor-172 (CI; apical addition at 10−5 M), and basal or apical addition of ATP (10−5 M). Pretreatment with CFTR inhibitor-172 did not have a significant effect on ATP-induced Isc. B: superimposed representative traces of Isc responses to basal and apical addition of 10−5 M ATP following treatment with 10−3 M FFA added to either the basal (dashed trace) or the apical (solid trace) bath. Am, 10−5 M amiloride (apical addition); FFA(b), 10−3 M FFA added to the basal bath; FFA(a), 10−3 M FFA added to the apical bath; ATP(b), 10−5 M ATP added to the basal bath; ATP(a), 10−5 M ATP added to the apical bath. C: average Isc values in response to basal addition of ATP (ΔIscATP) to control cells (open bars), cells pretreated with FFA added to the apical side (closed bars), and cells pretreated with FFA added to the basal side (dashed bars). Values are represented as means ± SE (n = 11 filters). D: average Isc values in response to apical addition of ATP to control cells (open bars), cells pretreated with FFA added to the apical side (closed bars), and cells pretreated with FFA added to the basal side (dashed bars). Values are represented as means ± SE (n = 14 filters). *, **, #Values significantly different from control values.