Fig. 2.

Effect of ERK1/2, AKT, p38, and JNK pathway inhibitors on necrotic RPTC-induced P2X7 expression in NRK-49F. NRK-49F were pretreated with LY294002 (20 μM), U0126 (20 μM), SP600125 (10 μM), or SB 203580 (10 μM) for 1 h and then incubated with necrotic RPTC supernatant for 24 h. A: cell lysates were prepared and subjected to immunoblot analysis with specific antibodies against P2X7 or GADPH. B: levels of P2X7 receptor were quantified by densitometry and normalized with GADPH. Data are represented as means ± SD. Means with different superscript letters are significantly different from one another (P < 0.05). C: photomicrographs illustrating P2X7 (green) and nuclear staining (blue) of NRK-49F after various treatments.