Fig. 3.

Different methods of media pH reduction promote increased HKα₂ protein abundance. A: media pH was adjusted as indicated by addition of HCl. B: experiment was repeated 3 times, and fold-increase in HKα₂ vs. extracellular pH is shown. C: cells were cultured in incubators set for 5 or 10% CO₂ to achieve a media pH of 7.4 or 7.0, respectively. D: values are means ± SE for fold-increase in HKα₂ at 10 vs. 5% CO₂ from 3 independent experiments. ***P < 0.001.