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Inhibition of carbonic anhydrase does not affect upregulation of HKα2 during increase in CO2. Cells were subjected to 24-h treatment in either 5 or 10% CO2 in the presence of vehicle or 100 μM acetazolamide. A: representative immunoblot of HKα2 and β-actin expression. B: values are means ± SE from 4 independent experiments. **P < 0.01.
