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Regulation of endogenous HKα2 expression in m-IMCD-3 cells by extracellular pH. m-IMCD-3 cells were grown to near confluency, arrested 24 h in serum-free media, then subject to reduced extracellular pH by reduction of media bicarbonate concentration for another 24 h. A: representative immunoblot analysis of pH effect on HKα2 expression. B: values are means ± SE of data derived from 3 independent experiments.
