Fig. 2.
ANG II activates intracellular AT1 receptors in myometrial cells. A–D: fluorescent images of fura-2 AM-loaded myometrial cells illustrating the levels of basal [Ca2+]i (left) during microinjection of ANG II (10−7 M, middle), alone (A), or in the presence of losartan (B), PD-123319 (C), or saralasin (D), and 6 min after injection (right). The fluorescence intensity scale (0–4) is illustrated on each panel and magnified in A (left). E: averaged traces from 6 experiments indicating the increase in [Ca2+]i produced by ANG II alone or in the presence of AT1 receptor antagonist, losartan, AT2 receptor antagonist (PD 123319), or of AT1 and AT2 receptors antagonist, saralasin; losartan and saralasin abolished the increase in [Ca2+]i produced by injection of ANG II. F: comparison of the increase in [Ca2+]i produced by ANG II in the absence and presence of losartan, PD-123319, and saralasin. *P < 0.05 compared with ANG II alone.