Fig. 2.
mTORC1 effector 4E-BP1 regulates the expression of SREBP2 and its target genes. (A) Western blot of lysates from HCT15 and SW620 colorectal carcinoma cells treated with varying doses of PP242 for 4 h. (B) Relative mRNA levels in HCT15 and SW620 cells after 18 h treatment with DMSO or 2 μM PP242. (C) NIH 3T3 cells were cotransfected with FLAG-4E-BP1M and Tet activator expression constructs and induced with 1 μg/mL doxycycline, and cell lysates were prepared for immunoblotting. (D) NIH 3T3 cells were transfected as in C and mRNA levels were analyzed by qRT-PCR. (E) 4E-BP1 immunoblot analysis from four different cell lines. (Right) Protein levels are quantified and expressed as normalized ratios (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant).