Fig. 5.

Hypoxia potentiates LPA₁ activity by inhibiting GRK2 expression. (A–D) GRK2 inhibitor-treated cortices (B) show an increase in mitotic displacement compared with control hemispheres (A) (n = 15 matched pairs). This effect is absent in Lpar1^−/− cortices (C and D) (n = 9 matched pairs). (E) Quantification of A–D. (F) qRT-PCR quantification of GRK2 and GRK5 transcript levels in ex vivo cortices under normoxia or hypoxia (n = 6). (G) Representative Western blot of GRK2 protein in normoxic and hypoxic cortices and (H) corresponding quantitative analysis (n = 4). (Scale bar: 50 μm.)