Fig. 4.

Cardiolipin becomes extractable in the isolated cell envelope or after lysozyme treatment of cells. (A) Lipid composition of the CMW extract of intact cells (lane 1) and the CMW extract of the isolated cell envelope (lane 2) as detected by TLC with CMW (65:25:1 vol/vol). Lanes 3–5 contain phospholipid standards. PG, phosphatidylglycerol; CL, cardiolipin. Phosphomolybdotungstate was used for detection. (B) ESI-MS profile of the major lipid band, migrating like CL, isolated from the TLC plate of CMW reextract of lysozyme-treated delipidated cells.