Table 1.

Quantitation of lipids from C. glutamicum (% dry weight)

	CMW extract of RMS-treated cells (all noncovalently bound lipids of the IM)	RMS extract of intact cells (all noncovalently bound lipids of the OM)	CMW extract of lysozyme-treated cells (peptidoglycan-embedded lipids)
PI + PIMs	∼0.5	∼0.18*	0.2 ± 0.03†
Cardiolipin	0.22 ± 0.01	—	2.51 ± 0.1
Phosphatidylglycerol	0.97 ± 0.2	—
TDM	—	0.5 ± 0.1	—
Unknown lipid 1	~0.14	—	—
Diacylglycerols	~0.13	—	~0.2
Unknown lipid 2	—	~0.15	—
Free mycolic acids with traces of FFAs	—	~0.46	—
Mycolic acids cleaved from proteins	—	0.35 ± 0.01	—
Fatty acids cleaved from proteins	—	~0.01	—

 Values are averages from at least six experiments.

*This is composed nearly entirely of PIM. The amount is only approximate.

^†This is entirely PIM.