Table 2.

Fatty acid residue contents of C. glutamicum cell envelope (mmol/100 g dcm)

	C12-20 FAs (based on GC-MS)	Corynomycolates (estimated based on radioactivity and GC-MS)
Covalently and noncovalently linked, in the entire  cell envelope (whole-cell alkaline hydrolysis)	9.69 ± 0.2	1.79 ± 0.3*
OM (RMS extract)	0.39 ± 0.2	1.69 ± 0.4†
Peptidoglycan embedded (released in CMW extract  after lysozyme treatment)	4.8 ± 0.7§	—
Covalently bound to the cell wall (released after  alkaline hydrolysis of lysozyme-treated delipidated cells)	Negligible	≥0.22‡
IM (CMW extract − RMS extract)	4.3 ± 0.6	0

 Values are averages from at least six experiments.

*Calculated based on a C12-20 FA-to-corynomycolate ratio of 5.4 ± 0.9.

^†Calculated based on a C12-20 FA-to-corynomycolate ratio of 0.23 ± 0.1 for this fraction.

^‡Actual values are expected to be slightly higher because of some loss of cells in preceding CMW extractions and lysozyme treatment steps.