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. 2011 Sep 6;108(37):15147-15151. doi: 10.1073/pnas.1112157108

Fig. 4.

Fig. 4.

Trp emission spectra of LacY mutants with reduced background Trp fluorescence. (A) LacY mutants with different total numbers of native Trp residues replaced with Tyr: 1, WT LacY with six native Trp residues; 2, mutant W78Y/W171Y/W223Y with three Trp replaced; 3, mutant W33Y/W78Y/W171Y/W223Y with four Trp replaced; 4, mutant N245W/W78Y/W171Y/W223Y with Trp in place of Asn245 and three Trp replaced. (B) Mutants with inner quenchers targeting native Trp residues: 1, WT LacY; 2, mutant A88H (Trp171); 3, mutant A155C (Trp 151); 4, mutant N166H (Trp33); 5, mutant N38C (Trp33). The locations of the Trp residues in LacY and positions of inner quenchers introduced are shown in Fig. S4.