Table 1.
Thermodynamic parameters for Munc18/syntaxin 1 interactions from M. brevicollis
| Munc18 interaction with | Kd, nM | ΔH°, kcal/mole | n |
| Syx1 (1-279) | 3.9 ± 0.6 | −36.7 ± 0.2 | 0.91 |
| Syx1 (1-265) | 5.0 ± 0.9 | −36.8 ± 0.3 | 0.95 |
| Syx1 (1-242) | 450.4 ± 77.3 | −9.6 ± 0.6 | 0.94 |
| Syx1 (1-199) | 558.7 ± 88.6 | −2.8 ± 0.3 | 0.92 |
| Syx1 (1-20) | — | — | — |
| Syx1 (20-279) | 64.1 ± 7.0 | −25.8 ± 0.4 | 0.91 |
| SNARE complex containing Syx1 (1-279) | 571.4 ± 62.6 | −3.0 ± 0.2 | 1.04 |
| SNARE complex containing Syx1 (200-279) | — | — | — |
The corresponding experimental ITC data are shown in Fig. S3. Note that no heat changes were detected when Syx1 (1-20) or the SNARE-complex containing Syx1 (200-279) were mixed with Munc18.