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. 2011 Aug 29;108(37):15468–15473. doi: 10.1073/pnas.1107156108

Fig. 4.

Fig. 4.

Pharmacologic modulation of SLC habituation and SLC response sensitivity in 5-dpf larvae. (A) Mean SLC habituation after a 15-min incubation in 1% DMSO vs. varying doses of MK-801 or ketamine. (B) Mean SLC responsiveness to 10 low-level, subthreshold acoustic stimuli after a 15-min incubation in 1% DMSO vs. 100 μM MK-801 or 500 μM ketamine/1% DMSO. (C–F) Mean SLC responsiveness (binned by responses to 10 successive stimuli) to above-threshold acoustic stimulation (C and E) at a 20-s ISI (prehabituation phase) and a 1-s ISI (habituation phase) and to 10 low-level, subthreshold stimuli (D and F) after a 15-min incubation in hydrastine (C and D) or 12-MDA (E and F) at varying concentrations. (G) Chemical structure of the tested myristic acid analogs. (H) Mean SLC habituation after a 15-min incubation in combinations of varying concentrations of NMDA, ketamine, and/or 12-MDA. The number of larvae are shown in the bars in A and B; n = 32 larvae per group for C–F and H. *P < 0.01; **P < 0.001, Student t test vs. the DMSO group or indicated control group. #P < 0.001 vs. additive effect of the 5-μM 12-MDA and 50-μM ketamine groups, Student t test. Error bars indicate SEM.