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. 2011 Sep 1;108(37):15396–15401. doi: 10.1073/pnas.1019612108

Fig. 3.

Fig. 3.

Temperature-compensated, kaiC-dependent damped oscillations in digA and pilH/rre7 expression under DD. (A) Temporal expression profiles of the digA and hspA genes in the WT and kaiABC-null (ΔkaiABC) strains under DD after the cells were transferred from 12 h in the light (L12D). Asterisk shows the second peak of the damped digA mRNA cycle observed in the WT strain. (B) Temporal digA expression profile under DD at different temperatures (37 °C, 30 °C, and 25 °C). Note that the standard temperature in all other experiments described in the text was 30 °C. (C) Densitometric analysis of the temporal digA expression profile under DD at different temperatures in two independent experiments. (D and E) The kaiBC, digA, and pilH/rre7 mRNA expression profiles (D) and the KaiC phosphorylation cycles (E) in the rpoD5-null (ΔrpoD5) mutant strain and the kaiCS157P;ΔrpoD5 double mutant strain. For the experiments shown in A and B, the short-period mutant strain was transferred to the dark at hour 10.5 (estimated subjective dusk = CT 12, considering its period length of 21 h) from the light, after two 12 h/12 h LD cycles, whereas the WT strain was transferred to the dark at hour 12 (CT 12).