Fig. 2.

BFT induces SMO-dependent ROS and DNA damage in T84 cells. (A) T84 cells were pretreated with or without 25 μM MDL 72527 before stimulation with 5 nM BFT for 6 h. Total cellular lysates were analyzed by quantitative fluorescent Western blotting and γ-H2A.x levels were normalized to β-actin as described in Materials and Methods. The presented data and fold changes in protein levels include two samples per treatment group representative of multiple independent experiments. (B) T84 cell lines stably expressing nontargeting (Scr) and SMO-specific (SMO) shRNA were stimulated with BFT. Intracellular ROS levels were measured via CM-H₂DCFDA fluorescence.