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Identification of DHFRL1 and alignment to DHFR. (A) 5′ RACE was performed to identify DHFR transcripts that encoded a mitochondrial DHFR isozyme. Using a primer complementary to DHFR sequence, two bands were obtained following electrophoresis. The bands were isolated, cloned, and sequenced revealing DHFRL1 as an expressed transcript. (B) The sequence of DHFR and DHFRL1 were aligned using ClustalW in Lasergene Megalign software. The sequences are 92% identical.
