Skip to main content
. 2011 Aug 24;108(37):15207–15212. doi: 10.1073/pnas.1105414108

Fig. 3.

Fig. 3.

The loss of heterochromatic marks accompanies telomere lengthening. (A) Mean H4k20me3 intensity for primary MEFs (passage 2), in vitro cultured ICM, cells from the 96-well plate, and established ES cells at passage 9. (Lower graphs) The H4k20me3 histograms for the same samples. Note that in the ICM as well as in the 96-well plate there are cells with low H4k20me3 signals. (B) Percentage of cells with less than 7 arbitrary units of H4k20me3 fluorescence. Note the portion of cells with low methylation signal in both the cultured ICM and the 96-well plate. (C) Colocalization of the H4k20me3 heterochromatic mark with telomeres in percentage for the samples described in A. (D) Representative images of telomeres and H4k20me3 signals for the samples described in A. (E) Mean H3k9me3 intensity and histograms for the samples described in A. (F) Percentage of cells with less than 7 arbitrary units of H3k9me3 fluorescence. Note the portion of cells with low methylation signal in the cultured ICM and the 96-well plate. (G) Percentage of colocalization of the H3k9me3 heterochromatic mark with telomeres for the samples described in A. (H) Representative images of telomeres and H3k9me3 signals for the samples described in A. n = number of ICM or 96-well plate colonies or independent ES, and primary MEF cultures. Arbitrary units of H4k20me3 fluorescence is plotted. (Scale bars, 10 μm.)