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. 2011 Aug 29;108(37):15300–15305. doi: 10.1073/pnas.1107425108

Fig. 2.

Fig. 2.

ydj1Δ cells are defective in transcription initiation. (A) Gene expression ability is measured as growth in SC-trp-ura using the pLAUR system. Serial dilutions assays show transformants of WT and ydj1Δ cells. (B) Northern analysis of GAL1 mRNA after galactose induction in WT and ydj1Δ. rRNA is shown as a loading control. Quantification is shown below in arbitrary units. Mean and SD of two independent experiments are depicted. (C) Chromatin immunoprecipitation of Rad3-TAP at the GAL1 promoter upon galactose addition in WT and ydj1Δ cells. The mean and SD of triplicate assays of two independent experiments are depicted at each point. A detail of early time points is shown. (D) ChIP with anti-RNAPII (details as in C). (E) ChIP of Ydj1-FLAG at the GAL1 promoter after induction of transcription (galactose) or under repression conditions (glucose). Mean and SD of three independent experiments are depicted at each point.