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ydj1Δ cells are defective in transactivation. (A) Immunodetection of phosphorylation at Ser118 of ER at basal levels (0 min) and after β-estradiol addition in WT and the TFIIH kinase-defective mutant kin28-ts16. Total ER protein (ER-α) and β-actin loading controls are shown. (B) Immunodetection of phosphorylation at Ser118 of ER at basal levels (0 min) and after β-estradiol addition in WT and in ydj1Δ. Total ER protein (ER-α) and β-actin loading controls are shown.
