Fig. 4.

TFIIH stoichiometry is altered in ydj1Δ cells. (A) Immunodetection of Tfb4-TAP and Rad3-TAP by using anti-TAP antibody in WT and in ydj1Δ crude extracts from cells grown to exponential phase in SC. β-Actin loading control is shown. (B) Coomassie gel comparing the purified TFIIH complex obtained by Tfb4-TAP immunoprecipitation in WT and in ydj1Δ strains. Proteins identified by MALDI-TOF are indicated. Additional proteins, such as Pse1 and Eft2 (“1”), Cdc19 and Vma2 (“2”), Tef2 (“3”), Psa1 (“4”), or Thd2/3 (“5”) were identified in both strains. (C) Immunodetection of Tfb2, Ssl1, and Tfb4-TAP in purified extracts from two independent purifications to determine their relative abundance. The ydj1Δ: WT ratios of the level of each protein relative to Tfb4 are shown on the right.