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. 2011 Jun 3;301(3):L275–L284. doi: 10.1152/ajplung.00043.2011

Fig. 2.

Fig. 2.

Inhibition of phosphatidylinositol-3,4,5 triphosphate (PI3)-kinase activity increases expression of SmMHC protein in smooth muscle tissues subjected to low mechanical load. A: immunoblot of extracts from 4 individual muscle tissues subjected to low load or high load for 6 h in the presence or absence of the PI3-kinase inhibitor LY294002. B: mean values for effects of mechanical load and PI3-kinase inhibitor on Akt phosphorylation and SmMHC expression. A and B: in muscles without PI3-kinase inhibitor (No treatment), high load increased SmMHC expression and decreased Akt phosphorylation at Ser 473. PI3-kinase inhibitor significantly inhibited Akt activation and significantly increased SmMHC expression in low load muscles, but it did not significantly affect SmMHC expression in high load muscles, although it caused further inhibition of Akt. Values are means ± SE (n = 7). *Paired values are significantly different, P < 0.05.