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. 2011 Jun 3;301(3):L275–L284. doi: 10.1152/ajplung.00043.2011

Fig. 5.

Fig. 5.

Expression of PINCH LIM1–2 inhibits mechanosensitivity of IL-13-induced Akt activation and suppression of SmMHC expression. A: immunoblots from extracts of 8 tracheal muscle tissues treated with PINCH LIM1–2 or sham treated, stimulated with IL-13 or not stimulated, and subjected to low or high mechanical load. B: immunoblots of anti-GFP immunoprecipitates from muscle tissues expressing EGFP-PINCH LIM 1–2 protein demonstrating expression of recombinant PINCH proteins. C: mean results for the effects of IL-13 and IL-13 plus PINCH LIM 1–2 on mechanosensitive Akt activity and SmMHC expression (n = 6). IL-13 stimulation did not suppress the mechanosensitivity of Akt phosphorylation or SmMHC expression, but PINCH LIM 1–2 expression abolished the mechanosensitivity of both SmMHC expression and Akt phosphorylation. Values are means ± SE. *Paired values are significantly different, P < 0.05.