Fig. 3.

Detection of Mre11 in mitochondria of primary renal cells by immunocytochemistry. Primary kidney cells were exposed for 2 h to high NaCl (final osmolality 500 mosmol/kg) or kept at 300 mosmol/kg. The cells were immunostained for Mre11 (green) and mitochondrial antigen (red). DNA was stained with DAPI (blue). Mre11 is present in mitochondria (colocalization) both at 300 and 500 mosmol/kg. When NaCl is high, some of the Mre11 in cytoplasm is separate from mitochondria, consistent with the Western blot analysis result shown in Fig 2.