Fig. 4.

In mouse kidney, Mre11 is mostly cytoplasmic in metabolically active mitochondria-rich cells where it colocalizes with mitochondria. Analysis of subcellular localization of Mre11 in normal mouse kidney. A: immunohistochemistry of Mre11 in paraffin sections of normal mouse kidney. The subcellular distribution of Mre11 differs between cell types. Mre11 is mostly in the cytoplasm of some cells, but mostly in the nucleus of other cells. B and C: immunocytochemistry of Mre11 in frozen sections from normal mouse kidney. Sections are immunostained with anti-Mre11 (green) and anti-mitochondrial antigen (red) antibodies. DNA is stained with DAPI (blue). B: images scanned sequentially at different wave lengths. C: merged image of B. Much of Mre11 colocalizes with mitochondria.