FIG. 1.
Inhibition of translocation of Mre11, Rad50 and Nbs1 does not protect against heat radiosensitization. U-1 melanoma cells either were irradiated with X rays or were heated for 10 min at 45.5°C after irradiation in the presence or absence of leptomycin B (LMB). LMB was dissolved in ethanol (ETOH), which was used as a control vehicle. Error bars represent SEM for the data from at least two or three experiments. The plating efficiencies of the unheated and heated cells were as follows: no heat (control), ETOH = 0.3 ± 0.028; no heat (control), LMB = 0.21 ± 0.026; heat only, ETOH = 0.013 ± 0.002; heat only, LMB = 0.006 ± 0.0006.