FIG. 3.

Lack of involvement of Rad50 in heat radiosensitization. HeLa CCL2 cells were transfected with scrambled RNA (scRNA) or SMART pool siRNA directed against Rad50 for 72 h at 37°C. Cells were then irradiated or irradiated and then heated for 2 h at 41.5°C prior to plating for clonogenic survival. Error bars represent the SEM for the data from at least two or three experiments. The plating efficiencies of the unheated and heated cells were as follows: siRNA, no heat = 0.345 ± 0.015; siRNA, heat only = 0.25 ± 0.005; scRNA, no heat = 0.33 ± 0.02; scRNA, heat only = 0.22 ± 0.008.