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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Radiat Res. 2011 Jun 23;176(3):323–332. doi: 10.1667/rr2594.1

FIG. 6.

FIG. 6

Heated-induced association between Hsp70 and Mre11. HeLa cells were heated for 2 h at 42.5°C and incubated at 37°C for various times, and then Mre11 was immunoprecipitated from whole cell lysates prior to SDS-PAGE. Representative immunoblots probed for Mre11 and Hsp70 are shown (panel A). To quantitatively assess the interaction between Mre11 and Hsp70 as a function of time after heating, immunoblots were analyzed and Hsp70 content was normalized against the Mre11 protein content of the unheated control (panel B). The change in the amount of Hsp70 protein immunoprecipitated with Mre11 increased with time after heating.