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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: J Am Chem Soc. 2011 Jul 20;133(31):12172–12184. doi: 10.1021/ja2041964

Figure 3.

Figure 3

In vitro inhibition of bacterial transcription catalyzed by T. aquaticus RNAP. Artificial transcription elongation complexes containing radioactively labeled 8-nt RNA primer were assembled on nucleic-acid scaffolds (C) using T. aquaticus RNAP core enzyme. Where indicated, reaction mixtures were supplemented with 25 (A) or 250 µM (B) of small-molecule inhibitors and transcription was initiated by the addition of NTPs. Reaction products were resolved by denaturing polyacrylamide gel-elctrophoresis and revealed by autoradioagraphy.