Table 2.
Retrograde labeling following injections of Fluoro-Gold
| Retrogradely labeled structures | Injection sites | ||||||
|---|---|---|---|---|---|---|---|
| VTA | RRF | PAGvl | SN | ||||
| med | mid | lat | c | r | |||
| Cortex | |||||||
| frontal association | o | xxx | xx | xxx | xxx | xxx | o |
| medial orbital | o | x | x | xx | xxx | o | o |
| lateral orbital | o | xx | x | xx | xxx | o | o |
| prelimbic | xxx | xxx | xx | xxx | xxx | o | o |
| infralimbic | xxx | xxx | xx | xxx | xxx | o | o |
| dorsal peduncular | xxx | xxx | xx | xx | xxx | o | o |
| anterior cingulate | xx | xxx | xx | xx | xx | o | o |
| agranular insular | x | xxx | xxx | xxx | xxx | xxx | xx |
| claustrum | xx | xx | x | xxx | xx | xxx | x |
| dysgranular insular | o | o | x | xxx | xx | xxx | o |
| granular insular | o | o | x | xxx | xx | xx | o |
| second somatosensory | o | o | o | xx | o | xx | xxx |
| primary somatosensory | o | o | o | xx | o | xx | xxx |
| second motor | o | o | o | xx | o | o | xx |
| primary motor | o | o | o | xx | o | o | xx |
| Basal ganglia | |||||||
| accumbens rostral pole | xxx | xx | o | xxx | xx | xx | x |
| accumbens medial shell | xxx | xxx | x | o | x | xxx | o |
| accumbens lateral shell | x | x | xx | xxx | xxx | xx | x |
| accumbens core | xxx | o | x | xxx | o | xxx | xxx |
| ventral pallidum | xxx | xxx | xx | xx | xxx | x | o |
| caudate-putamen (mainly v and vl) | x | o | o | xxx | xx | xxx | xxx |
| globus pallidus | xx | x | o | xxx | xx | xxx | xxx |
| entopeduncular nucleus | o | xx | x | o | xx | xx | o |
| subthalamic nucleus | o | o | o | xxx | o | xxx | xxx |
| substantia nigra compacta | xxx | xx | xx | xxx | xxx | xxx | xx |
| substantia nigra reticulata | x | xx | xx | xxx | xxx | xxx | xx |
| Septum and extended amygdala | |||||||
| lateral septum | xxx | xx | xx | o | x | o | o |
| medial septum-diagonal band | xxx | xx | xx | o | x | o | o |
| bed nucleus of stria terminalis, med-d | xx | xxx | xxx | xxx | x | xxx | o |
| bed nucleus of stria terminalis, lat-d | x | x | x | xxx | xxx | xxx | o |
| bed nucleus of stria terminalis, med-v | xx | xxx | xxx | xxx | x | xx | o |
| bed nucleus of stria terminalis, lat-v | x | xxx | xxx | xxx | xxx | xx | o |
| marginal nucleus of Shu et al. (1988) | o | o | o | xxx | o | xxx | xxx |
| sublenticular extended amgydala | xxx | xxx | xxx | xxx | xxx | xxx | xx |
| anterior amygdaloid area | xxx | xxx | xxx | xxx | xxx | xxx | xx |
| central amygdaloid nucleus, lat | xx | xxx | xxx | xxx | o | xxx | x |
| central amygdaloid nucleus, med | xx | xxx | xxx | xxx | xxx | xxx | xx |
| Hypothalamus | |||||||
| median preoptic nucleus | xx | xx | x | o | xxx | o | o |
| lateral preoptic area | xxx | xxx | xx | xxx | xxx | xxx | o |
| medial preoptic area | xxx | xxx | xxx | xxx | xxx | xxx | o |
| lateral hypothalamic area | xxx | xxx | xxx | xxx | xxx | xxx | o |
| paraventricular nucleus | xx | xx | xx | xxx | xxx | xx | o |
| posterior hypothalamic area | xxx | xxx | xx | o | xxx | x | o |
| central gray | xxx | xxx | xxx | xxx | xxx | xxx | xxx |
| supramammillary nucleus | xxx | xx | o | o | xxx | o | o |
| mammillary body | x | xx | o | o | o | o | o |
| Thalamus and epithalamus | |||||||
| lateral habenula | xxx | xxx | xx | xx | xxx | xxx | o |
| parafascicular nucleus | xxx | xxx | xxx | xxx | xxx | xxx | o |
| ventromedial nucleus | x | xxx | xx | xx | o | xx | xx |
| posterior intralaminar nucleus | xx | xxx | x | xx | xxx | xx | xx |
| Other structures (ordered rostrocaudally) | |||||||
| zona incerta | xxx | xxx | xxx | xxx | xxx | xxx | xx |
| parasubthalamic nucleus | x | x | o | xxx | xxx | xxx | o |
| ventral tegmental area | xx | xx | x | xx | xxx | xx | xx |
| interpeduncular nucleus | nd | x | o | o | o | o | o |
| rostromedial tegmental nucleus | xxx | x | xx | xxx | xx | xxx | xx |
| superior colliculus, deep layers | xx | xxx | xxx | xxx | xx | xxx | xx |
| periaqueductal gray | xxx | xxx | xxx | xxx | xxx | xxx | xx |
| deep mesencephalic nucleus | xx | xxx | xxx | xxx | xxx | xxx | xx |
| dorsal raphe | xxx | xxx | xx | xx | xxx | xx | xx |
| pedunculopontine tegmental nucleus | xxx | xxx | xxx | xxx | xxx | xxx | xx |
| laterodorsal tegmental nucleus | xxx | xxx | xxx | xx | xxx | xx | x |
| median raphe | xxx | xx | o | x | xx | o | o |
| parabrachial nucleus | xx | xxx | xxx | xxx | xxx | xxx | xx |
| cuneiform nucleus | xxx | xx | xxx | xxx | xxx | x | xx |
| pontine reticular nucleus, oralis | xx | xxx | xx | xxx | xxx | xx | x |
| pontine reticular nucleus, caudalis | x | xxx | xx | xxx | xxx | xx | x |
| principal nucleus V, ventrolateral (contralat) | o | xx | x | xx | xx | xx | o |
| Evaluated cases | 99039 | 08024 | 06108 | 05103 | 10098 | 05104 | 05096 |
| 99060 | 06157 | 05105 | 10099 | 05106 | 05095 | ||
| 06166 | 10090 | ||||||
| 10091 | |||||||
| 10096 | |||||||
| 10097 | |||||||
Representative injection sites are illulstrated in Figure 1. o = absent or trace retrograde labeling reflecting 0–5 labeled cells/structure. + = 6 to 15 labeled neurons/section. ++ = 16–50 labeled neurons/section. +++ = more than 50 labeled neurons/section/structure. This list references labeling of ipsilateral structures following the indicated injections, listed in approximate rostocaudal sequence. Representative contralateral labeling is illustrated in Figure 3. Abbreviations: c – compacta; d- dorsal; lat – lateral; lat-d – lateral division, dorsal part; lat-v – lateral division, ventral part; med – medial; med-d – medial division, dorsal part; med-v – medial division ventral part; mid – middle; PAG – periaqueductal gray; RRF – retrorubral field; SN – substantia nigra; v – ventral; vl – ventrolateral; VTA – ventral tegmental area.