Fig. 10.
Inducing low-frequency activity reveals an early LEV effect. A: experimental protocol for each choice of activity augmentation: 4-aminopyridine (4-AP) perfusion, 0.2-Hz stimulation, or 10-Hz stimulation. B, C, and D: averages of the normalized amplitudes of the 18th through 20th EPSCs of 80-Hz trains for slices incubated for 30 min in control or 300 μM LEV ACSF with either 4-AP perfusion (B), 0.2-Hz stimulation (C), or 10-Hz stimulation (D). The horizontal reference line marks the comparable average from slices treated 30 min with 300 μM LEV. All data shown are means ± SE (n = nos. shown in bar diagrams are from at least 3 different animals per group; P values from Student's t-test). *,**Significant differences.