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Cholinergic axons innervate arcuate nucleus. Cholinergic axons were identified with immunostaining against the vesicular acetylcholine (ACh) transporter VAChT. Red VAChT immunoreactive axons and boutons (arrows) surround a neuron expressing green fluorescent protein (GFP) under regulation of the proopiomelanocortin (POMC; A) and neuropeptide Y (NPY; B) promoter. Scale bar, 6 μm.
